Renal Physiology Lab Reporting Worksheet

Renal Physiology Lab Reporting Worksheet

In science, reporting what has been done in a laboratory setting is incredibly important for communicating, replicating, and validating findings. However, writing scientific reports can be a little overwhelming. There is a set of agreed-upon components that the scientific community requires when reporting scientific experimentation. Answer the following questions to describe what occurred during the lab you conducted in Labster. Be sure to use complete sentences and descriptions that fully represent what you experienced. Writing a lab report is less about being correct or incorrect, than it is accurately reporting what happened and why. So, do not worry about reporting data that might seem counterintuitive or unexpected. Focus on clearly communicating what you did and what you observed.

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TITLE:

What was the title of the lab you completed?

Renal Physiology Lab

PROBLEM:

What was the problem you were trying to resolve in the Lab?

The problem in the lab was to understand the process of renal physiology, specifically focusing on filtration and reabsorption in the renal nephron.

HYPOTHESIS:

What information from the textbook and classroom is relevant for the problem you were trying to resolve in the lab? Identify the concepts and explain how they are related to the lab problem.

In the Renal Physiology Lab, the relevant information from the textbook and classroom was about renal physiology, specifically focusing on filtration and reabsorption in the renal nephron. The concepts discussed included the function of the nephron, glomerular filtration, tubular reabsorption, and factors influencing the reabsorption rate.

The concepts relevant to the lab include:

Nephron Function: The nephron is the functional unit of the kidney responsible for filtration, reabsorption, and secretion. The nephron is related to the lab problem because it helps understand the structure of nephron and its function, which is crucial to comprehend how the renal system works.

Tubular Reabsorption: After filtration, some substances like water, glucose, and ions are reabsorbed from the renal tubules back into the blood. Tubular Reabsorption is closely attached to the lab problem because it helps in regulating the body’s fluid and electrolyte balance.

Glomerular Filtration: This refers to the process where blood is filtered through the glomerulus, a network of capillaries in the nephron. Filtration is related to the lab problem because it allows water and solutes to pass from the blood into the renal tubules.

Based on the information from the textbook and classroom, I hypothesized that the reabsorption rate of the renal tubule would be influenced by the concentration of the perfusate saline and the presence of a radioactive agent.

During the lab, what information from the THEORY section provided additional background information about the problem? (To review the theory section, launch the lab and click the “Theory” tab at the top of the data pad). Identify the concepts and explain how they are related to the lab problem.

In the laboratory, the theoretical part offered further contextual details concerning the mechanism of straining and assimilation inside the nephron of the kidney. This explained kidney structure, how nephrons function, glomerular filtration rate, and the function of antidiuretic hormones in controlling urine production.

Most scientific experimentation involves examining variables and their relationships. A variable is a construct that can be changed and studied. Examples of variables are a condition or measurable quantity. What are the variables you examined in the lab? Which one were you controlling and changing? Which one were you observing was impacted by your change?

Variables:

The concentration of the Perfusate Saline: This variable was manipulated to study its influence on the reabsorption rate in the renal tubule.

Presence of Radioactive Agent: This variable was used to investigate its effect on the reabsorption rate in the renal tubule.

In the experimental setup, I accurately controlled and manipulated the concentration of the radioactive compound present in the perfusate saline. By systematically altering the concentration levels, I keenly observed and recorded its impact on the volume of liquid reabsorbed through the renal tubule. This comprehensive analysis allowed me to establish a clear cause-and-effect relationship between the concentration of the compound and the observed changes in the reabsorption of liquid.

Developing a hypothesis requires understanding relevant background knowledge. Now that you have described relevant background information, it is time to develop a hypothesis. A hypothesis is a simple statement (not a question) grounded in previous research that predicts the relationship between the variables being studied. Please make a statement that predicts the relationship between the variables being studied.

An increase in the concentration of the perfusate saline would result in a decrease in the reabsorption rate of the renal tubule.

METHOD:

Describing what you did during a lab helps other scientists to replicate your work. It is through this consistent replication that scientists are able to see repeating patterns and develop ideas that help move science forward. When you discuss your data, in a later section, you will have to describe what choices you made, why you made them, and any concerns about things that occurred that were unexpected. In order to have enough information to do this, you need to keep very detailed notes. What doesn’t seem important at the moment may end up being something that explains your findings later. A benefit of conducting virtual labs when learning science is that many potential errors are controlled for you. The virtual lab environment often will alert you if something is not going the way it should. This does not occur in non-virtual settings. For this reason, the virtual lab setting can be very helpful to learners. However, we still have to practice documenting so that those skills can be practiced for lab experiences when technology is not there as a coach.

You have already, identify the variables that you studied in the lab in the previous section. Now, take some time to fully define and describe what each variable is and how it was changed throughout the lab.

During the lab, I examined the variables of filtration and reabsorption in the renal nephron. I controlled and changed the concentration of the radioactive compound in the perfusate saline. I observed that the volume of liquid being reabsorbed through the epithelial walls of the renal tubule was impacted by the change in concentration of the radioactive compound.

In 3-5 sentences, summarize what you did during the lab, not including your process of logging into the system. This section would be much more robust for a non-virtual lab. For this virtual lab, a short, high-level summary will suffice.

During the virtual lab, I followed the procedure below:

I followed the given instructions and prepared the perfusate saline. I then carefully inserted the pipettes into the renal tubule. To follow this, I carefully measured and recorded the volume collected in the collecting pipette. Finally, I looked over and examined the data gathered to make predictions based on the outcomes that were seen.

Describe some of the observations you made. What numbers did you write down or keep track of? What did each of your senses observe during the lab process? What did you see (ex: changes in colors, movement, shapes, sizes, patterns)? What did you hear (ex: sounds from reactions, collisions, error messages)? What did your lab character touch? Did you notice anything that seemed unexpected? Did you notice anything that you did not expect to observe?

What I saw:

During the simulation, I observed the liquid in the collecting pipette. There were no changes in colors, movement, shapes, sizes, or patterns as expected, as the focus was on measuring the volume of liquid reabsorbed. Consistently, I didn’t hear any sounds from reactions, collisions, or error messages. It was a straightforward measurement process.

During the lab, I observed and recorded the volume of liquid collected in the collecting pipette. We also made note of any unexpected observations or events that occurred during the experiment. I did not expect to observe any significant changes in color, movement, or shape, as the focus was on measuring the volume of liquid reabsorbed.

Which parts of the lab required you to think more than others and required more time? Which parts were simple and completed easily?

The parts of the lab that required more thinking and time were setting up the apparatus, ensuring accurate measurements, and interpreting the results. The steps involved in manipulating the pipettes and accurately measuring the volumes required meticulous attention and focus. On the other hand, the process of injecting the perfusate saline and collecting the liquid was relatively straightforward and completed effortlessly.

DATA & RESULTS:

Many lessons learned as a result of scientific experiments come from the reporting and analysis of data. This part of scientific reporting requires detailed descriptions of technical information and quantities as well as high-level synthesis of information. High-level synthesis requires a mastery of foundational content in the related scientific field and a complimentary mastery in some fields of quantitative and/or qualitative analysis. For this report, let’s focus on big-picture patterns.

What relationships did you notice between the variables you examined? When you changed the variable(s), how did the other(s) change?

In this observation, I noticed that when the radioactive compound concentration increased, the amount of liquid collected in the pipette decreased. This shows that reabsorption happens more often at higher concentrations. This finding suggests that there might be important reasons behind this relationship, which gives a better understanding of how reabsorption works when higher concentrations occur. By further investigating and analyzing these results, I can learn valuable information about how the radioactive compound behaves and interacts with the system. This discovery can significantly impact related areas and encourage scientists to study these areas in detail.

Did you notice any patterns in your data? Any patterns between the variables?

I observed a pattern of active transport mechanisms involved in the renal tubule’s reabsorption of ions and water.

DISCUSSION:

The discussion section is used to explain why things might have happened the way that they did in your experiment. Here, scientists describe any potential anomalies or mistakes and why they think they may have occurred.

During your lab, what happened that might have impacted the accuracy of your data? Did the simulation alert you that an error was occurring? If so, how did you resolve it?

Because I was measuring small amounts, I made various data collection mistakes during the lab. The simulation alerted me to potential errors, and I resolved them by carefully remeasuring the volumes and concentrations. Through careful attention to detail and precise calibration of the measuring equipment, I ensured that the collected data was more accurate and reliable. This approach allowed me to change any differences and obtain reliable results for further analysis. By taking the necessary corrective actions, I was able to enhance the overall validity and integrity of the experimental data, leading to more meaningful conclusions and insights from the research.

CONCLUSION:

The conclusion section of a lab report describes how the learnings from the lab experimentation fit into prior scientific knowledge. This is done by comparing new information to previously known information that was identified in the section of your report that discusses background information.

Review the hypothesis section of your report from above and describe how the results of your lab compare to the background information that you discussed before.

The lab experiment results matched the information in the theory part and observed the process of electrically charged particles and fluid inside the tubules of the kidney. It was discovered that active transport processes, such as primary and secondary active transport, are crucial for the specific process. The information from the test not only verified something I already understood but also provided me with new details about how these mechanisms function. This assisted in comprehending more effectively how renal functions and sustains the proper stability of aqueous substances in the human body. These labs improved my knowledge of renal physiology and how the renal tubule efficiently reabsorbs ions and water to keep the body in balance.

Once scientists have identified how the new knowledge fits into the old knowledge, they discuss the implications of the new information for moving forward. In this class, the purpose of the study is to learn some foundational science ideas represented by the course student learning outcomes. Review the course student learning outcome aligned with this lab in the assignment directions on Blackboard. How is the information from this lab related to the course student learning outcome? What knowledge has the lab supported you with learning that is related to this course’s student learning outcome?

The lab supported my learning related to the course student learning outcome of understanding renal physiology and the mechanisms of filtration and reabsorption in the kidney.

Following scientific experimentation, scientists usually come up with new questions that result from what they learned. These new questions often end up leading to new experiments in the future. What additional scientific things do you wonder about after completing and writing about your lab experience?

How do variations in osmolarity or hormone levels affect the reabsorption process in the renal tubule?

How do specific drugs or substances impact the efficiency of active transport mechanisms in the nephron?

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Creating a lab reporting worksheet for renal physiology involves several key components. Here’s a structured approach to designing one:

Title and Introduction:

Provide a clear title that indicates the purpose of the lab.

Write a brief introduction explaining the objectives of the lab and its relevance to renal physiology.

Materials and Methods:

List all the materials and equipment used in the experiment.

Describe the experimental procedure step by step, including any measurements taken and techniques employed.

Experimental Setup:

Include a diagram or description of the experimental setup, highlighting the key components such as the kidney model, tubing, and fluid reservoirs.

Data Collection:

Create tables or spaces for recording raw data obtained during the experiment.

Include parameters such as urine flow rate, glomerular filtration rate (GFR), urine osmolality, and any other relevant measurements.